Clinical evaluation of three chromogenic media for the isolation of Staphylococcus aureus in respiratory samples in patients with cystic fibrosis.

Diagn Microbiol Infect Dis

French National Reference Centre for Staphylococci, Hospices Civils de Lyon, Lyon, France; CIRI - Centre International de Recherche en Infectiologie, Université de Lyon, Inserm U1111, CNRS UMR5308, Ecole Normale Supérieure de Lyon, Université Claude Bernard Lyon 1, Lyon, France; Laboratoire de Bactériologie, Institut des Agents Infectieux, Hospices Civils de Lyon, France.

Published: January 2021

AI Article Synopsis

  • - The study compared the effectiveness of three chromogenic media (BBL CHROMagar™ Staph aureus, ChromID™ S. aureus SAID, ChromID™ S. aureus Elite SAIDE) and CNA media for isolating Staphylococcus aureus from respiratory samples of cystic fibrosis patients.
  • - All four media had a similar capability of supporting S. aureus growth, with sensitivity improving when samples were incubated for over 24 hours.
  • - Among the tested media, SAIDE showed the highest sensitivity while maintaining good specificity even after 72 hours of incubation.

Article Abstract

We evaluated the performance of three chromogenic media (BBL CHROMagar™ Staph aureus, ChromID™ S. aureus SAID, ChromID™ S. aureus Elite SAIDE) for the isolation of Staphylococcus aureus in respiratory samples in patients with cystic fibrosis in comparison with CNA media. We reported a similar ability of the four media to support the growth of S. aureus and that sensitivity increased when incubation lasted more than 24 h. SAIDE had the higher sensitivity compared to the other media and kept a high specificity even after 72 h.

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http://dx.doi.org/10.1016/j.diagmicrobio.2020.115201DOI Listing

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