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Aim: To study the role of iodine, selenium and zinc in the pathogenesis of iodine deficiency and autoimmune thyroid diseases and scientifically substantiate the choice of security biomarkers and analytical methods for determination.
Materials And Methods: Serum iodine (I), selenium (Se), and zinc (Zn) concentrations were measured using inductively coupled plasma ionization tandem mass spectrometry (Agilent 8900 ICP-MS Triple Quad); by chemiluminescent immunoassay on an automatic analyzer Architect i2000 - TSH and AT-TPO in blood serum; by enzyme immunoassay - ZnT8A; biochemical method - ALP, SOD1 in 1150 people aged from 18 to 65 years (the average age of the subjects was 40±5 years). Ultrasound of the thyroid gland was performed in the supine position using a portable ultrasound machine LOGIQe with a multifrequency linear sensor 10-15 MHz; during the study, the volume of the thyroid gland, the presence of nodules and their characteristics according to the TIRADS classification, the structure of the thyroid gland and its echogenicity were assessed.
Surveillance of hoof disorders in Korean dairy cattle and the correlation of farm condition risk factors to their prevalence.
BMC Vet Res
March 2025
Department of Animal Science and Technology, Sunchon National University, 255 Jungang-ro, Suncheon-si, Jeollanam-do, 57922, South Korea.
Background: This study investigated the prevalence of hoof disorders (HDs) in intensive dairy farms in Korea and their association with farm conditions. A total of 877 cattle from 15 farms were examined for infectious, noninfectious, and non-lesion HDs at the animal, foot, and farm levels. Risk factors such as bedding depth, floor wetness, floor elevation transitions, and aggressive hoof treatment were evaluated.
View Article and Find Full Text PDFEffect of delayed isolation of peripheral blood mononuclear cells on cell viability and functionality.
BMC Immunol
March 2025
Department of Gynecology and Obstetrics, Universitätsklinikum Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), Erlangen, Germany.
Background: Peripheral blood mononuclear cells (PBMCs) are valuable biomarkers, providing crucial insights into the patients' immune system. Reliable biobanking of PBMCs is essential to minimize heterogeneity. In multicenter trials, blood sample transportation to central laboratories can increase the time between blood collection and PBMC isolation.
View Article and Find Full Text PDFThe usefulness and potential of high-fidelity three-dimensional models in spine surgery training: cross-sectional empirical study.
BMC Med Educ
March 2025
Department of Orthopaedic Surgery, Dokkyo Medical University, 880, Kitakobayashi, Mibu, Shimotsuga, Tochigi, Japan.
Background: Advancements in understanding spinal disorders and diagnostic techniques have increased the range and complexity of spinal surgeries. However, constraints have arisen in gaining experience techniques through actual surgical cases due to considerations of medical safety, efficiency in working hours, and cost-effectiveness. As such, off-the-job training is expected to play an increasingly significant role.
View Article and Find Full Text PDFRestorative management of permanent teeth affected by molar incisor hypomineralization: a survey of dentists in Saudi Arabia.
BMC Oral Health
March 2025
Pediatric Dentistry Department, Faculty of Dentistry, King Abdulaziz University, PO Box 80200, Jeddah, 21589, Saudi Arabia.
Background: This electronic survey-based study aimed to explore the variations in treatment decisions made by dentists from different specialties in Saudi Arabia when treating anterior and posterior permanent teeth afflicted by molar incisor hypomineralization (MIH) of varying severity.
Methods: A cohort of dental professionals, including general dentists, pediatric dentists, and restorative dentists/prosthodontists, participated in a validated electronic survey-based study between January and March 2023. The survey consisted of the following three sections: The first section collected demographic information from the participating dentists.
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