AI Article Synopsis
- Axolotls are known for their impressive ability to regenerate various organs, including limbs and hearts, but liver regeneration had not been studied until now.
- The study used techniques such as block-face imaging and histology to analyze liver regeneration after partial hepatectomy, revealing a different structure and regeneration process compared to other vertebrates.
- The results indicate that while axolotls increase the number of proliferating liver cells, they do not exhibit complete liver shape recovery, suggesting their regeneration mechanism is based on compensatory congestion rather than traditional epimorphic regeneration.
Article Abstract
Background: Axolotls have remarkable organ-level regeneration capability. They can regenerate their limbs, tail, brain, gills, and heart. The liver had been considered to be a regenerative organ in these highly regeneration-competent animals. Therefore, no research had been performed on liver regeneration in urodele amphibians. In the present study, we focused on axolotl liver regeneration and found a unique regeneration mechanism compared with other vertebrates.
Results: Partial hepatectomy (PH) was performed to assess axolotl liver regeneration. Regeneration was assessed using block-face imaging (CoMBi), histology, cell proliferation, weight gain, and Albumin (Alb) + area. Axolotl liver histology was compared with other vertebrates. Axolotl liver consists of Glisson's capsule, sinusoids, and hepatic cord with no apparent lobule structures. Hepatocytes were mononucleated or multinucleated. PH increased the multinucleated hepatocytes and the Alb + area, but there was no apparent liver shape recovery even 40 days after PH. Gene expression pattern suggests that no epimorphic regeneration takes place. We also found that the increase in the number of proliferating hepatocytes was regulated by ERK-signaling.
Conclusion: Our findings suggest that axolotls, which have epimorphic regeneration ability, regenerate their liver via unique mechanisms, compensatory congestion.
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| http://dx.doi.org/10.1002/dvdy.262 | DOI Listing |
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