Rapid identification of methicillin-sensitive (MSSA), heterogeneous vancomycin-intermediate (hVISA), and vancomycin-intermediate (VISA) is important for accurate treatment, timely intervention, and prevention of outbreaks. Here, 90 isolates were analyzed for protein biomarker discovery, including MSSA, vancomycin-susceptible (VSSA), hVISA, and VISA strains. Label-free data-independent acquisition proteomics was used to identify protein biomarkers that allow for discrimination among MSSA, hVISA, and VISA strains. There were 8786 nonredundant peptides identified, corresponding to 418 different annotated nonredundant proteins. Two VISA protein biomarkers, two hVISA protein biomarkers, and one MSSA protein biomarker with high sensitivities and specificities were discovered and verified. Data are available via MassIVE with identifier MSV000085776.
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