AI Article Synopsis
- C21 steroidal glycosides have been shown to be effective in treating various cancers, and this study focused on a synthesized C21 fraction's effects on gastric cancer cell lines.
- Significant growth inhibition, cell cycle arrest, and increased apoptosis were observed in gastric cancer cells treated with the C21 fraction, particularly when combined with the autophagy inhibitor chloroquine.
- The study highlights a novel mechanism for C21's anti-tumor activity, emphasizing its ability to trigger apoptosis by altering the balance of pro- and anti-apoptotic proteins and increasing reactive oxygen species production.
Article Abstract
C21 steroidal glycosides have been extensively reported for treating several types of cancer and are widely found in . In this study, a C21 fraction was synthesized from , and its anti-cancer potency was assessed against in vitro gastric cell lines BGC-823, SGC-7901, and AGS. Significant growth inhibition and cell cycle arrest were observed in C21 fraction-treated gastric cancer cells. The results of apoptotic staining techniques in C21 fraction-treated gastric cells were confirmed with excess reactive oxygen species generation. Moreover, SOD and HO levels were increased by C21 fraction, especially when combined with chloroquine (CQ). The apoptotic inducing potential of C21 fraction was also evidenced by upregulation of proapoptotic proteins cleaved-PARP and BAX and downregulation of antiapoptotic proteins Bcl-2 and p-AKT by western blot, especially in the presence of the autophagy inhibitor CQ. The results showed that the apoptosis of gastric cancer cells caused by C21 fraction was enhanced by inhibiting autophagy. The current findings reveal a new mechanism for the antitumor activity of C21 fraction on gastric cancer.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7542599 | PMC |
| http://dx.doi.org/10.1021/acsomega.0c02748 | DOI Listing |
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