The periodontal pathogen Porphyromonas gingivalis produces gingipains (Kgp, RgpA, and RgpB), cysteine proteases involved in the organism's virulence, and pigmentation. We previously showed that deletion of the PGN_0297 and PGN_0300 genes reduced the proteolytic activity of gingipains. The role of the PGN_0296 gene, consisting of an operon with the PGN_0297 and PGN_0300 genes, is unclear. Herein, we examined the effect of PGN_0296 gene deletion on the proteolytic activity. Although the proteolytic activity of the gingipains did not decrease in the culture supernatant of a PGN_0296 gene deletion mutant (ΔPGN_0296), the growth was delayed.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.job.2020.09.007 | DOI Listing |
Publication Analysis
Top Keywords
proteolytic activity
12
pgn_0296 gene
12
porphyromonas gingivalis
8
pgn_0297 pgn_0300
8
pgn_0300 genes
8
activity gingipains
8
gene deletion
8
construction characterization
4
pgn_0296
4
characterization pgn_0296
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!