AI Article Synopsis
- - Six known pathways for carbon monoxide (CO) fixation exist in microorganisms, with D. desulfuricans using a new, seventh pathway involving hydrogen and sulfate as energy sources.
- - This reductive glycine pathway converts CO to formate, which is then transformed into glycine and finally into acetyl-CoA, eventually generating pyruvate.
- - The pathway's efficiency in generating ATP highlights the role of ammonia, as the growth rate of D. desulfuricans is affected by ammonia concentration, showcasing its autotrophic capabilities.
Article Abstract
Six CO fixation pathways are known to operate in photoautotrophic and chemoautotrophic microorganisms. Here, we describe chemolithoautotrophic growth of the sulphate-reducing bacterium Desulfovibrio desulfuricans (strain G11) with hydrogen and sulphate as energy substrates. Genomic, transcriptomic, proteomic and metabolomic analyses reveal that D. desulfuricans assimilates CO via the reductive glycine pathway, a seventh CO fixation pathway. In this pathway, CO is first reduced to formate, which is reduced and condensed with a second CO to generate glycine. Glycine is further reduced in D. desulfuricans by glycine reductase to acetyl-P, and then to acetyl-CoA, which is condensed with another CO to form pyruvate. Ammonia is involved in the operation of the pathway, which is reflected in the dependence of the autotrophic growth rate on the ammonia concentration. Our study demonstrates microbial autotrophic growth fully supported by this highly ATP-efficient CO fixation pathway.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7547702 | PMC |
| http://dx.doi.org/10.1038/s41467-020-18906-7 | DOI Listing |
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