, as an effective model of cereal grains, is susceptible to most destructive cereal pathogens. () has been studied extensively in . is one of the important regulators of plant immunity. However, no homologous genes of were found in . In this study, the gene was transformed into Three transgenic plant lines containing the gene were confirmed by PCR and GUS gene activity. There were fewer urediospores in the -overexpressing plants compared to wild type plants. biomass was obviously decreased in transgenic plants. The length of infection hyphae and infection unit areas of were significantly limited in transgenic plants. Moreover, there were small lesions in transgenic plants challenged by . Salicylic acid accumulation was significantly increased, which led to elevated gene expression in transgenic inoculated by or compared to wild type plants. These results were consistent with infected phenotypes. Overexpression of in caused resistance to and . These results suggest that could regulate plant resistance in .
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http://dx.doi.org/10.3390/plants9101316 | DOI Listing |
Plant Mol Biol
January 2025
College of Agronomy, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
The lipoxygenase (LOX) gene family is widely distributed in plants, and its activity is closely associated with seed viability and stress tolerance. In this study, we cloned the rice(Oryza sativa)lipoxygenase gene OsLOX1, a key participant in the 13-lipoxygenase metabolic pathway. Our primary focus was to investigate its role in mediating responses to drought stress and seed germination in rice.
View Article and Find Full Text PDFNat Med
January 2025
Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of MIT and Harvard, Cambridge, MA, USA.
Prion disease is a fatal neurodegenerative disease caused by the misfolding of prion protein (PrP) encoded by the PRNP gene. While there is currently no cure for the disease, depleting PrP in the brain is an established strategy to prevent or stall templated misfolding of PrP. Here we developed in vivo cytosine and adenine base strategies delivered by adeno-associated viruses to permanently modify the PRNP locus to achieve PrP knockdown in the mouse brain.
View Article and Find Full Text PDFNat Commun
January 2025
State Key Laboratory of Plant Trait Design, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences (CAS), Shanghai, 200032, China.
Cyclic nucleotide-gated channel 5 (CNGC5), CNGC6, and CNGC9 (CNGC5/6/9 for simplicity) control Arabidopsis root hair (RH) growth by mediating the influx of external Ca to establish and maintain a sharp cytosolic Ca gradient at RH tips. However, the underlying mechanisms for the regulation of CNGCs remain unknown. We report here that calcium dependent protein kinase 1 (CPK1) directly activates CNGC5/6/9 to promote Arabidopsis RH growth.
View Article and Find Full Text PDFProc Natl Acad Sci U S A
January 2025
Department of Economics, University of Oregon, Eugene, OR 97403.
The advent of herbicide-tolerant genetically modified (GM) crops spurred rapid and widespread use of the herbicide glyphosate throughout US agriculture. In the two decades following GM-seeds' introduction, the volume of glyphosate applied in the United States increased by more than 750%. Despite this breadth and scale, science and policy remain unresolved regarding the effects of glyphosate on human health.
View Article and Find Full Text PDFGM Crops Food
December 2025
School of Life Science, Henan University, Kaifeng, Henan, People's Republic of China.
Malic acid markedly affects watermelon flavor. Reducing the malic acid content can significantly increase the sweetness of watermelon. An effective solution strategy is to reduce watermelon malic acid content through molecular breeding technology.
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