Achromobacter aestuarii sp. nov., Isolated from an Estuary.

A novel strain KS-M25 was isolated from estuary water in South Korea. Strain KS-M25 was Gram-staining-negative, strictly aerobic, motile rods-shaped bacterium and showed oxidase- and catalase-positive reactions. Growth of strain KS-M25 was observed at 10-25 °C (optimum, 20 °C), at pH 5.5-9.0 (optimum, pH 7.5), and with 0-6.0% (w/v) NaCl (optimum, 1%). Ubiquinone-8 was identified as the sole isoprenoid quinone and the major fatty acids were C cyclo-C and sum in feature 3 (comprising C ω7c and/or C ω6c). The G+C content values based on genome sequences was 62.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain KS-M25 formed a clear cluster within the genus Achromobacter. Strain KS-M25 was most closely related to Achromobacter anxifer LMG 26857, Achromobacter dolens LMG 26840, and Achromobacter xylosoxidans NBRC 15126 with 97.8%, 97.8%, and 97.7% 16S rRNA gene sequence similarities, respectively. Based on phenotypic, chemotaxonomic, and phylogenetic analysis, strain KS-M25 represents a novel species of the genes Achromobacter, for which the name Achromobacter aestuarii sp. nov. is proposed. The type strain is KS-M25 (= KACC 21219 = JCM 33329).