Metallo-beta-lactamase-producing spp. is a major challenge for therapeutic treatment of nosocomial infections. This study is aimed at determining the prevalence of MBL-producing spp. among 87 clinical isolates of spp. from the Korle-Bu Teaching Hospital, Accra, between August 2014 and July 2015. spp. was identified by standard bacteriological method, and resistance to different antibiotics was assessed with the Kirby-Bauer disc diffusion method. Meropenem-resistant isolates were screened for enzyme activity using the modified Hodge test (MHT) and combined disc test (CDT). Additionally, multiplex PCR was used to determine MBL genes presence (VIM IMP, and NDM). All isolates showed high resistance to cefotaxime (90.8%), ceftazidime (75.9%), cotrimoxazole (70.1%), ciprofloxacin (64.4%), gentamicin (72.4%), levofloxacin (67.8%), and meropenem (59.8%). A total of 54 (62.1%) of isolates were multidrug-resistant. Out of 52 (59.8%) meropenem-resistant , 3 (5.8%) were carbapenemase producers by MHT, whilst, 23 (44.2%) were CDT positive. There was no significant difference between the resistance pattern of amikacin, ceftazidime, cotrimoxazole, ciprofloxacin, and meropenem amongst CDT-positive and CDT-negative isolates ( > 0.05). A total of 7/87 (8.1%) CDT-positive isolates harboured NDM; of these, 4 (57.1%) were from wound swabs, urine ( = 2) (28.6%), and ear swab ( = 1) (14.3%). The study revealed that less than 9% of spp. contained NDM encoding genes. Strict antibiotics usage plan and infection control measures are required to prevent the spread of these resistance genes.
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| http://dx.doi.org/10.1155/2020/3852419 | DOI Listing |
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