AI Article Synopsis
- Autophagy is a critical process for cellular degradation, but its specific role in how cells adapt to changes, like shifting to respiratory growth, isn't well understood.
- Research shows that in yeast (Saccharomyces cerevisiae), autophagy helps recycle serine, a crucial one-carbon metabolite, which is necessary for effective mitochondrial function during respiratory growth.
- When autophagy is defective, yeast cells experience delays in growth and mitochondrial processes, but adding serine can restore proper mitochondrial protein expression and support quicker adaptation to using respiration for energy.
Article Abstract
The mechanism and function of autophagy as a highly-conserved bulk degradation pathway are well studied, but the physiological role of autophagy remains poorly understood. We show that autophagy is involved in the adaptation of Saccharomyces cerevisiae to respiratory growth through its recycling of serine. On respiratory media, growth onset, mitochondrial initiator tRNA modification and mitochondrial protein expression are delayed in autophagy defective cells, suggesting that mitochondrial one-carbon metabolism is perturbed in these cells. The supplementation of serine, which is a key one-carbon metabolite, is able to restore mitochondrial protein expression and alleviate delayed respiratory growth. These results indicate that autophagy-derived serine feeds into mitochondrial one-carbon metabolism, supporting the initiation of mitochondrial protein synthesis and allowing rapid adaptation to respiratory growth.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7542147 | PMC |
| http://dx.doi.org/10.1038/s41467-020-18805-x | DOI Listing |
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