Exceedingly Sensitive Restructured Electrodes Design for Pathogen Morphology Detection using Impedance Flow Cytometry.

The cellular morphology is a vital biological characteristic for determining explicit information about its physiological state. Monitoring real-time cell shape is of great importance in infectious pathogen detection. Here, we designed a highly sensitive coplanar electrode sensing system and merged it with planar electrodes for simultaneous impedance signals in two dimensions. We simulated the proposed design in this study for the detection of different single cell pathogens based on their morphology. The optimized design has a great potential to monitor and characterize different bacteria based on their sizes and shapes. In this report, spherical and rod shaped particles were used to illustrate the device performance. This simple and extremely sensitive modified electrode design is very promising for bacterial detection and will serve as a future guiding tool for discriminating different morphologies of singular cells.