AI Article Synopsis
- Nasopharyngeal colonization is essential for the spread and illness caused by pneumococcus, but existing vaccines only target a few serotypes, leading to potential serotype replacement.
- Researchers analyzed metal concentrations in human nasal fluid to understand how these levels affect bacterial growth and protein expression.
- They found that pneumococci grown with nasal fluid-like metal concentrations expressed distinct proteins, identifying AliA as a strong protective antigen that could be a promising candidate for future vaccines.
Article Abstract
Nasopharyngeal colonization by is a prerequisite for pneumococcal transmission and disease. Current vaccines protect only against disease and colonization caused by a limited number of serotypes, consequently allowing serotype replacement and transmission. Therefore, the development of a broadly protective vaccine against colonization, transmission and disease is desired but requires a better understanding of pneumococcal adaptation to its natural niche. Hence, we measured the levels of free and protein-bound transition metals in human nasal fluid, to determine the effect of metal concentrations on the growth and proteome of . Pneumococci cultured in medium containing metal levels comparable to nasal fluid showed a highly distinct proteomic profile compared to standard culture conditions, including the increased abundance of nine conserved, putative surface-exposed proteins. AliA, an oligopeptide binding protein, was identified as the strongest protective antigen, demonstrated by the significantly reduced bacterial load in a murine colonization and a lethal mouse pneumonia model, highlighting its potential as vaccine antigen.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7550026 | PMC |
| http://dx.doi.org/10.1080/21505594.2020.1825908 | DOI Listing |
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