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http://dx.doi.org/10.1021/acssynbio.0c00477 | DOI Listing |
Photosynth Res
July 2023
Institute of Microbiology of the Czech Academy of Sciences, Novohradská 237 - Opatovický Mlýn, 37901, Třeboň, Czech Republic.
ACS Synth Biol
October 2020
Department of Biomedical Engineering, Boston University, Boston, Massachusetts 02215, United States.
Mutat Res
December 1998
Center for Marine Research, Rudjer Boskovic Institute, Rovinj, Croatia.
The marine demosponge Geodia cydonium encodes a gene, termed GCXPB, which displays 62% identity to the human XPB/ERCC-3 gene that specifically corrects the repair defect in xeroderma pigmentosum and in Cockayne's syndrome. The cDNA was isolated and characterized the deduced aa sequence, XPB_GEOCY, with the calculated size of 91,541 Da comprises the characteristic domains found in the related helicases. Phylogenetic tree analysis revealed that the sponge sequence is grouped to the metazoan related XPB/ERCC-3 polypeptides.
View Article and Find Full Text PDFEur J Biochem
August 1992
Institut für Botanik, Universität Hannover, FRG.
An in-vitro system has been established to study the integration of early light-inducible proteins (ELIP) into isolated thylakoid membranes. The in-vitro-expressed ELIP precursor proteins exist in two forms, a high-molecular-mass aggregate which is accessible to trypsin but no longer to the stromal processing protease and a soluble form which is readily cleaved to the mature form by the stromal protease. The mature form of ELIP is integrated into thylakoid membranes; its correct integration can be deduced from the observation that the posttranslationally transported products and the in-vitro integrated ELIP species are cleaved by trypsin to products of the same apparent molecular mass.
View Article and Find Full Text PDFPlant Cell
October 1990
Max-Planck-Institut für Züchtungsforschung, Köln, Federal Republic of Germany.
An elicitor-regulated transient expression system was established in soybean protoplasts that allowed the identification of cis-regulatory elements involved in plant defense. The 5' region of an ultraviolet (UV) light-inducible and elicitor-inducible chs gene (chs1) of soybean was subjected to deletion analysis with the help of chimeric chs-nptII/gus gene constructs. This analysis delimited the sequences necessary for elicitor inducibility to -175 and -134 of the chs1 promoter.
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