AI Article Synopsis
- Chromatin immunoprecipitation followed by next-generation sequencing (ChIP-seq) is a technique used to understand how proteins bind to DNA across the entire genome.
- The ChIP-seq workflow needs to be tailored for different sample types to ensure high-quality results.
- This specific protocol is aimed at human embryonic stem cells and includes methods for verifying sample quality and minimizing non-specific binding before sequencing, as detailed in Gunne-Braden et al. (2020).
Article Abstract
Chromatin immunoprecipitation (ChIP) followed by next-generation sequencing is a powerful technique that characterizes the genome-wide DNA-binding profile of a protein of interest. The general ChIP-seq workflow has been applied widely to many sample types and target proteins, but sample-specific optimization of various steps is necessary to achieve high-quality data. This protocol is specifically optimized for cultured human embryonic stem cells (hESCs), including steps to check sample quality and non-specific enrichment of "hyper-ChIPable" regions prior to sequencing. For complete details on the use and execution of this protocol, please refer to Gunne-Braden et al. (2020).
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7501726 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2020.100062 | DOI Listing |
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