AI Article Synopsis

  • Conventional antimicrobial treatments are becoming less effective due to rapid multidrug resistance, leading to the need for alternative methods to control infections.
  • Antimicrobial photodynamic therapy (aPDT) shows promise as a treatment for polymicrobially infected wounds, tested through both lab models and live mouse experiments.
  • Results indicate that aPDT's effectiveness depends on the environment, and it significantly improves wound healing in infected mice with proper application of methylene blue and LED light.

Article Abstract

Rapidly evolving multidrug resistance renders conventional antimicrobial strategies increasingly inefficient. This urges the exploration of alternative strategies with a lower potential of resistance development to control microbial infections. A promising option is antimicrobial photodynamic therapy (aPDT), especially in the setting of wound infections. In this study its effectiveness was tested as a treatment option for polymicrobially infected wounds in both in vitro and in vivo models. First, aPDT was applied to wound-relevant Gram-positive and Gram-negative bacteria in planktonic culture as the standard in vitro test system and compared different media to show a possible dependency of the therapy on the surrounding environment. In a second step, aPDT was investigated in an in vitro model mimicking the wound bed conditions using fibrin-coated culture plates. Finally, we tested aPDT in vivo in a polymicrobial infected wound healing model in immunocompromised BALB/c mice. In vitro, it was shown that the bactericidal effectiveness of aPDT was strongly dependent on the surrounding environment of the phototoxic reaction. In vivo, the significant delay in wound healing induced by polymicrobial infection was drastically diminished by a two-times application of aPDT using 100 μM methylene blue (generally regarded as safe for topical application on human skin) and 24 J cm pulsed red LED light. Our experiments suggest that aPDT is capable of significantly improving wound healing also in complicated polymicrobially infected wound situations.

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http://dx.doi.org/10.1039/d0pp00108bDOI Listing

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