Background: L., a perennial oilseed plant, is considered as a promising feedstock for biodiesel production. Genetic modification of flowering characteristics is critical for breeding. However, analysis of floral-specific promoters in is limited.
Methods: In this study, we isolated the ortholog of () gene from flower cDNA library and detected the expression pattern of gene by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). We isolated a 1.8-kb fragment from the 5' region of the gene and evaluated its spatiotemporal expression pattern in using the - () reporter gene and () gene, respectively.
Results: was identified as a flower-specific gene in . As expected, promoter was only active in transgenic flowers with the strongest activity in stamens. Moreover, transgenic showed a phenotype of large flowers without any alterations in other organs. Furthermore, deletion of the region from -1,717 to -876 bp resulted in the disappearance of promoter activity in stamens but an increase in promoter activity in young leaves, sepals, and petals. Deletion analysis suggests that the -1,717- to -876-bp promoter fragment contains regulatory elements that confer promoter activity in stamens and inhibit activity in young leaves, sepals, and petals.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7502236 | PMC |
| http://dx.doi.org/10.7717/peerj.9827 | DOI Listing |
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