Background: Anaplasma and Ehrlichia species are tick-borne pathogens of both veterinary and public health importance. The current status of these pathogens, including emerging species such as Ehrlichia minasensis and Anaplasma platys, infecting cattle in Kenya, remain unclear, mainly because of limitation in the diagnostic techniques. Therefore, we investigated the Anaplasma and Ehrlichia species infecting dairy cattle in Nairobi, Kenya using molecular methods.
Results: A total of 306 whole blood samples were collected from apparently healthy dairy cattle. Whole blood DNA was extracted and tested for presence of Anaplasma and Ehrlichia DNA through amplification and sequencing of the 16S rDNA gene. Sequence identity was confirmed using BLASTn analysis while phylogenetic reconstruction was performed to determine the genetic relationship between the Kenyan isolates and other annotated genotypes available in GenBank. Anaplasma and Ehrlichia species were detected in 19.9 and 3.3% of all the samples analyzed, respectively. BLASTn analysis of the sequences against non-redundant GenBank nucleotide database revealed infections with A. platys (44.8%), A. marginale (31%) and A. bovis (13.8%). All four sequenced Ehrlichia spp. were similar to Ehrlichia minasensis. Nucleotide polymorphism was observed for A. platys, A. bovis and E. minasensis. The Anaplasma species clustered in four distinct phylogenetic clades including A. marginale, A. platys, A. bovis and some unidentified Anaplasma spp. The Kenyan Ehrlichia minasensis clustered in the same clade with isolates from America and Australia but distant from E. ruminantium.
Conclusion: This study provides the first report of infection of dairy cattle in Kenya with A. platys and E. minasensis, which are emerging pathogens. We conclude that cattle in peri-urban Nairobi are infected with various species of Anaplasma and E. minasensis. To understand the extent of these infections in other parts of the country, large-scale screening studies as well as vector identification is necessary to inform strategic control.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7526178 | PMC |
| http://dx.doi.org/10.1186/s12917-020-02584-0 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Tick-borne pathogens from ruminant ticks in a Mediterranean ecosystem from Eastern Spain.
Med Vet Entomol
December 2024
Department of Animal Production and Public Health, Faculty of Veterinary Medicine and Experimental Sciences, Universidad Católica de Valencia San Vicente Mártir, Valencia, Spain.
Vector-borne diseases represent various sicknesses that are increasingly significant in human and veterinary health. Among the zoonotic agents transmitted by ticks, infections caused by the intracellular pathogens from the Anaplasmataceae family and piroplasmids (Babesia/Theileria spp.) are particularly notable due to the substantial economic losses they cause in the livestock sector.
View Article and Find Full Text PDFTicked Off: A Case of Disappearing Cell Lines.
Cureus
November 2024
Internal Medicine, Weiss Memorial Hospital, Chicago, USA.
Human granulocytic anaplasmosis (HGA) is transmitted by the black-legged tick and presents with fever, thrombocytopenia, leukocytopenia, and elevated transaminases. If left untreated, HGA can progress to hemophagocytic lymphohistiocytosis (HLH), which can be fatal. Here, we discuss a case of a woman diagnosed with anaplasmosis who was treated promptly.
View Article and Find Full Text PDFCanine Vector-Borne Diseases (CVBDs) in Liguria, North-West Italy: A Retrospective Study over an 11-Year Period (2013-2023).
Animals (Basel)
December 2024
S.S. Genova e Portualità Marittima, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Piazza Borgo Pila 39-24, 16129 Genova, Italy.
Canine vector-borne diseases (CVBDs) pose a global threat to both canine and public health. This study evaluates the prevalence of spp., spp.
View Article and Find Full Text PDFMolecular detection and characterization of Anaplasmataceae agents, Bartonella spp. and hemoplasmas in armadillos and anteaters from Brazil.
Acta Trop
December 2024
Vector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction, and One Health, School of Agricultural and Veterinary Sciences, São Paulo State University "Júlio de Mesquita Filho" (FCAV/UNESP), Jaboticabal Campus, Via de Acesso Prof. Paulo Donato Castellane, s/n, Rural Zone, Jaboticabal, SP, CEP: 14884-900, Brazil. Electronic address:
Article Synopsis
- Armadillos and anteaters interact with various pathogens and parasites, making them potential hosts for zoonotic diseases, but research on these interactions is limited.
- A study in Brazil analyzed 167 samples from different species of armadillos and anteaters to identify occurrences of pathogens like Ehrlichia, Anaplasma, Bartonella, and hemoplasmas.
- Results showed low detection rates, with only one positive sample each for Anaplasma in six-banded armadillos and Ehrlichia in giant anteaters, indicating the need for further research on disease dynamics in these mammals.
Exploring tick-borne pathogens in community dogs in Nepal.
Parasitol Int
November 2024
Laboratory of Parasitology, Graduate School of Infectious Diseases, Faculty of Veterinary Medicine, Hokkaido University, Japan; Division of Parasitology, Veterinary Research Unit, International Institute for Zoonosis Control, Hokkaido University, Japan. Electronic address:
Article Synopsis
- Tick-borne pathogens (TBPs) in dogs pose a significant global health threat, particularly in developing countries where surveillance is lacking.
- A study involving 230 community dogs in Nepal found that 32.6% were infected with at least one TBP, with common pathogens including Ehrlichia canis and Anaplasma platys.
- The findings suggest that infections in community dogs might pose a risk for transmission to pet dogs and humans, highlighting the need for increased awareness and control measures against these zoonotic parasites.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!