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Luminescence Properties of Hoechst 33258 in Polyvinyl Alcohol Films.
Int J Mol Sci
January 2025
Department of Physics and Astronomy, Texas Christian University, Fort Worth, TX 76129, USA.
We report a comprehensive investigation of the photophysical properties of Hoechst 33258 (HOE) embedded in polyvinyl alcohol (PVA) films. HOE displays a bright, highly polarized, blue fluorescence emission centered at 430 nm, indicating effective immobilization within the polymer matrix of PVA. Its fluorescence quantum yield is notably high (~0.
View Article and Find Full Text PDFBiodegradation of Phenol at High Initial Concentration by 3D Strain: Biochemical and Genetic Aspects.
Microorganisms
January 2025
Laboratory of Microbial Enzymology, G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, Prosp. Nauki 5, 142290 Pushchino, Russia.
Phenolic compounds are an extensive group of natural and anthropogenic organic substances of the aromatic series containing one or more hydroxyl groups. The main sources of phenols entering the environment are waste from metallurgy and coke plants, enterprises of the leather, furniture, and pulp and paper industries, as well as wastewater from the production of phenol-formaldehyde resins, adhesives, plastics, and pesticides. Among this group of compounds, phenol is the most common environmental pollutant.
View Article and Find Full Text PDFCytochrome P450 electrochemical biosensors transforming in vitro metabolism testing - Opportunities and challenges.
Bioelectrochemistry
January 2025
LAQV-REQUIMTE, Laboratory of Applied Chemistry, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal. Electronic address:
The ability of the living world to flourish in the face of constant exposure to dangerous chemicals depends on the management ability of a widespread group of enzymes known as heme-thiolate monooxygenases or cytochrome P450 superfamily. About three-quarters of all reactions determining the metabolism of endogenous compounds, of those carried in foods, of taken drugs, or even of synthetic chemicals discarded into the environment depend on their catalytic performance. The chromatographic and (photo)luminometric methods routinely used as predictive and analytical tools in laboratories have significant drawbacks ranging from limited shelf-life of reagents, use of synthetic substrates, laborious and tedious procedures for highly sensitive detection.
View Article and Find Full Text PDFNatural exposure to Chikungunya virus in golden-headed lion tamarin (Leontopithecus chrysomelas, Kuhl, 1820) from non-protected areas in southern Bahia, Brazil: Implications and significance.
PLoS Negl Trop Dis
January 2025
Department of Agricultural and Environmental Sciences, Universidade Estadual de Santa Cruz, Ilhéus, Bahia, Brazil.
Chikungunya virus (CHIKV) is primarily associated with non-human-primates (NHPs) in Africa, which also infect humans. Since its introduction to Brazil in 2014, CHIKV has predominantly thrived in urban cycles, involving Aedes aegypti mosquitoes. Limited knowledge exists regarding CHIKV occurrence and implications in rural and sylvatic cycles where neotropical NHPs are potential hosts, from which we highlight Leontopithecus chrysomelas (Kuhl, 1820), the golden-headed lion tamarin (GHLT), an endangered species endemic to the Atlantic Forest (AF) in Southern Bahia State, Brazil.
View Article and Find Full Text PDFOptimizing extrusion-based 3D bioprinting of plant cells with enhanced resolution and cell viability.
Biofabrication
January 2025
Mechanical Engineering, Tsinghua University, A421 Lee Shau Kee Building, Tsinghua Uniersity, Haidian District, Beijing, 100084, CHINA.
3D bioprinting of plant cells has emerged as a promising technology for plant cell immobilization and related applications. Despite the numerous progress in mammal cell printing, the bioprinting of plant cells is still in its infancy and needs further investigation. Here, we present a systematic study on optimizing the 3D bioprinting of plant cells, using carrots as an example, towards enhanced resolution and cell viability.
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