A novel ratiometric fluorescent probe for selective detection and imaging of HS.

In this work, a novel phenoxazine-based fluorescent probe BPO-N was developed to detect HS. The results showed that the probe had high selectivity and sensitivity toward HS, and its detection mechanism was based the ratio between green and red fluorescence signals; its detection limit was as low as 30 nM. The fluorescent imaging experiments further showed that the probe BPO-N could successfully detect endogenous and exogenous HS in living cells. This probe can be used as a powerful tool for in-depth study of HS function in various physiological processes.