AI Article Synopsis
- Chronic lymphocytic leukemia (CLL) affects T-cell function, leading to weakened immune responses, with Tim-3 serving as a critical checkpoint on exhausted T-cells during tumor development.
- The study compared the expression of Tim-3, Fyn, and Bat3 mRNA in peripheral blood mononuclear cells from 54 CLL patients and 34 healthy controls, using techniques like cDNA synthesis and Real-Time PCR.
- Results showed that while Tim-3 levels were similar in both groups, Fyn expression was lower and Bat3 was higher in CLL patients, highlighting a significant link between Tim-3 and Fyn, suggesting their combined role in promoting T-cell exhaustion in CLL.
Article Abstract
Background: Chronic lymphocytic leukemia (CLL) is correlated with defects in T-cell function resulting imparity in antitumor immune responses. Tim-3 is a co-inhibitory immune checkpoint receptor expressed on exhausted T-cells during tumor progression. Fyn and Bat3 are two important adaptor molecules involved in inhibition and activation of Tim-3 downstream signaling, respectively. In this study, the expression of Tim-3, Fyn, and Bat3 mRNA was evaluated in CLL patients.
Methods: Peripheral blood mononuclear cells (PBMCs) were isolated from 54 patients with CLL and 34 healthy controls. Total RNA was extracted from all samples and applied for cDNA synthesis. The relative expression of Tim-3, Fyn, and Bat3 mRNA was determined by TaqMan Real-Time PCR using GAPDH as an internal control.
Results: Tim-3 mRNA expression was not significantly different between CLL patients and healthy controls. Fyn mRNA expression was significantly lower in CLL patients and conversely, Bat3 mRNA expression was higher in CLL patients compared to healthy controls. Interestingly, the mRNA expression of Fyn inhibitory adaptor molecule was remarkably associated with expression of Tim-3 in CLL patients.
Conclusion: We have highlighted for the first time the expression of Fyn and Bat3 adaptor molecules in CLL patients. Our data demonstrated the strong correlation between the expression of Tim-3 and Fyn inhibitory molecules in CLL implying an important role for Tim-3-Fyn cooperation in induction of T-cell exhaustion.
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Source |
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7779459 | PMC |
| http://dx.doi.org/10.31557/APJCP.2020.21.9.2615 | DOI Listing |
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