Introduction: The article describes the occurrence and phylogenetic relationship of isolates found in subcutaneous abscesses of leopard geckos. The aim of the study was to determine the cause of the abscesses and to characterise isolated strains.
Material And Methods: Samples of abscesses from five animals and internal organs (lungs, liver, and gut) of three of them were tested for according to the PN-EN ISO 6579:2002/A1:2007 standard. The antimicrobial resistance was evaluated by minimal inhibitory concentrations and the genetic similarity of the isolates was assessed with pulsed field gel electrophoresis (PFGE).
Results: In total, seventeen isolates belonging to five different serovars were found to be susceptible to all tested antimicrobials except streptomycin. The serovars were . Hadar, . Fluntern, . Tennessee, subsp. 55:k:z, and . Kentucky. Up to three serovars from different organs were isolated from the same individual. In two geckos, were detected in the lungs. In three serovars, I-PFGE typing revealed indistinguishable isolates from organs and abscesses.
Conclusion: Multiple serovars might be involved in abscess formation and infections. The occurrence of the same PFGE profiles of the isolates may testify to the role of opportunistic organisms in causing infection.
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| http://dx.doi.org/10.2478/jvetres-2020-0058 | DOI Listing |
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