Growth comparison of genotypes T3 and T4 in several culture media.

causes severe diseases such as Granulomatous Amebic Encephalitis (GAE) and keratitis (AK). Improving the culture media classically used for this amoeba could help to identify it quickly and facilitate its study as a biological model. The purpose of this study was to compare the growth of two genotypes (T3 and T4) in several culture media. (T3 genotype) and (T4 genotype) were cultured in PYG, TSY, TYI-S-33, RPMI, and RPMI-FBS medium. The number of amoebas grown in different culture media was counted and compared to each other for 14 days. Findings in this research revealed the highest growth in RPMI-FBS medium. For this reason, we can recommend this culture medium to promote the growth of in its biological studies.