AI Article Synopsis
- Osteosarcoma (OS) is a prevalent bone tumor, and the role of circular RNA circ_0001721 in its progression is yet to be understood, despite its known influence on various cancers.
- Researchers employed multiple techniques like qRT-PCR, glucose consumption tests, and transwell assays to analyze the expression of circ_0001721 and its impact on osteosarcoma cell behaviors, including proliferation and invasion.
- Results showed that circ_0001721 and MAPK7 levels are increased in OS tissues while miR-372-3p is decreased; knockdown of circ_0001721 reduced glycolysis and cancer cell activity, indicating that circ_0001721 acts as a sponge for miR-372-3
Article Abstract
Background: Osteosarcoma (OS) is the most common bone tumor. Many studies have reported that circular RNAs (circRNAs) play an important role in the development of a variety of human cancers. However, the underlying mechanism of circ_0001721 in regulating osteosarcoma progression remains unknown.
Materials And Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the levels of circ_0001721, miR-372-3p, and mitogen-activated protein kinase 7 (MAPK7) in osteosarcoma tissues and cells. Besides, glycolysis was investigated by glucose consumption, lactate production and hexokinase II (HK2) protein level. Cell proliferation and apoptosis were determined by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) and flow cytometry, separately. Cell migration and invasion were determined by transwell assay. Moreover, the protein levels of HK2 and epithelial-to-mesenchymal transition (EMT) markers were determined by Western blot analysis. The relationship between miR-372-3p and circ_0001721 or MAPK7 was predicated by starbase v3.0 and confirmed by dual-luciferase reporter assay or RNA binding protein immunoprecipitation (RIP) assay. Murine xenograft model was established to investigate the role of circ_0001721 in vivo.
Results: The levels of circ_0001721 and MAPK7 were upregulated in osteosarcoma tissues and cells, while miR-372-3p was downregulated. Knockdown of circ_0001721 inhibited glycolysis, cell proliferation, cell migration, invasion and epithelial-to-mesenchymal transition (EMT), and promoted apoptosis. Circ_0001721 was validated as a sponge of miR-372-3p and mediated glycolysis, cell proliferation, apoptosis, migration, invasion, and EMT of osteosarcoma cells through miR-372-3p. MAPK7 was a target of miR-372-3p and overexpression of MAPK7 attenuated anti-cancer role of miR-372-3p in OS cells. Further studies revealed that circ_0001721 regulates MAPK7 expression via sponging miR-372-3-p. Finally, knockdown of circ_0001721 inhibited tumor progression in vivo.
Conclusion: Circ_0001721 promoted osteosarcoma development through the miR-372-3p/MAPK7 axis.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7498501 | PMC |
| http://dx.doi.org/10.2147/CMAR.S244527 | DOI Listing |
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