The synergy of porous substrates and functional genera for efficient nutrients removal at low temperature in a pilot-scale two-stage tidal flow constructed wetland.

A pilot-scale two-stage tidal flow constructed wetland (TFCW) with working volume of 0.46 m/d packing with shale ceramsite (SC) and activated alumina (AA) was constructed (named as SC-AA-TFCW) for nutrients removal at low temperature (<15 °C). SC-AA-TFCW achieved stable removals of 78.1% nitrogen and 98.3% phosphorous. SC-TFCW contributed to 55.2% of organics and 85.6% of particulate phosphorous removal. Among 17 denitrifiers, the absolute abundance of aerobic denitrification bacteria (ADNB) was highest, followed by facultative anaerobic denitrification bacteria (FADNB) and autotrophic denitrification bacteria (AUDNB). Nitrogen assimilating into organic nitrogen, dissimilatory and assimilatory nitrate reduction and complete denitrification may be main nitrogen metabolic pathways. Some ADNB (e. g. Zoogloea, Pseudomonas and Acidovorax) showed positive interactions with various key functional genes related to nutrients removal. Dissolved oxygen and reducing elements were main environmental factors in changing ADNB compositions. This study highlights the importance of ADNB and their synergy to porous substrates in SC-AA-TFCW.