AI Article Synopsis
- Co-culturing chondrocytes with mesenchymal stromal cells (MSCs) enhances cartilage tissue engineering by promoting chondrocyte growth and matrix production through trophic actions.
- MSCs allow for the creation of larger cartilage constructs using fewer chondrocytes.
- The chapter includes various methods for co-culturing MSCs and chondrocytes and detailed protocols for analyzing these co-cultures through different scientific techniques.
Article Abstract
Co-culture of chondrocytes and mesenchymal stromal cells (MSCs) has been shown to be beneficial in engineering cartilage tissue in vitro. In these co-cultures, MSCs increase the proliferation and matrix deposition of chondrocytes. The MSCs accomplish this beneficial effect by so-called trophic actions. Thus, large cartilage constructs can be made with a relatively small number of chondrocytes. In this chapter, we describe different methods for making co-cultures of MSCs and chondrocytes. We also provide detailed protocols for analyzing MSC-chondrocyte co-cultures with cell tracking, proliferation assays, species-specific polymerase chain reactions (PCR), rheological analysis, compression analysis, RNA-sequencing analysis, short tandem repeats analysis, and biochemical examination.
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| http://dx.doi.org/10.1007/978-1-0716-0989-7_5 | DOI Listing |
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