AI Article Synopsis
- The study examined how the tumour suppressor microRNA miR-214 affects gene expression in cervical cancer cells, specifically comparing HPV-positive (CaSki) and HPV-negative (C33A) cells.
- The investigation involved knocking out or overexpressing miR-214 and analyzing gene expression through RNA sequencing, revealing 904 upregulated and 365 downregulated genes between the two types of cancer cells with significant changes.
- Eleven key genes with a fold change of +/-5 were further validated using real-time PCR, marking the first exploration of miR-214's influence on gene expression related to HPV.
Article Abstract
In this study we have investigated the effects of a tumour suppressor microRNA, miR-214, on gene expression in HPV-positive (CaSki) and HPV-negative cervical cancer cells (C33A) by RNA sequencing using next generation sequencing. The HPV-positive and HPV-negative cervical cancer cells were either miR-214- knocked-out or miR-214-overexpressed. Gene expression analysis showed that a total of 904 genes were upregulated and 365 genes were downregulated between HPV-positive and HPV-negative cervical cancer cells with a fold change of +/- 2. Furthermore, 11 differentially expressed and relevant genes which showed a fold change of +/-5 were selected to confirm by real-time PCR. This study represents the first report of miR-214 on global gene expression in the context of HPV.
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