Growth and metabolic characterization of four lactic acid bacteria species isolated from rice beer prepared in Assam, India.

Isolation and identification of lactic acid bacteria (LAB) from rice beer prepared in Assam, India was performed and their growth associated and functional properties were studied. LAB strains were identified as , , and based on 16 s rRNA sequencing. Their growth characteristics at different pH, NaCl concentration, temperature and presence of carbohydrates were profiled. High tolerance against acid and bile salts was shown by all the strains, particularly TEZU174 and TEZU199 up to a pH of 1.5, and TEZU174 up to 14  % bile concentration. They were susceptible towards the common antibiotics, wherein erythromycin, chloramphenicol and linezolid were the most effective. The strains displayed antibiosis activity against and and antioxidant activity in terms of resistance to HO, scavenging of ·OH and DPPH free radicals was also displayed, wherein TEZU374 and TEZU482 were the most effective with above 70  % scavenging activity. The strains displayed cellular aggregation and TEZU262 and TEZU309 were highly aggregated, which attained 100  % autoaggregation within a period of 5 h. High cell surface hydrophobicity was shown by TEZU309 towards xylene and chloroform, and TEZU427 towards ethyl acetate. The strains evinced good gut tolerance capacity, antioxidant activity and adherence properties, which are characteristics of probiotic bacteria and thus are candidates for therapeutic uses and also to be used as starter cultures.