AI Article Synopsis
- The pSETT4 vector uses the φC31 integrase to integrate into the sp. SE50/110 chromosome for efficient gene cloning.
- It employs Golden Gate assembly with the BsaI restriction enzyme to enable easy insertion of the gene of interest.
- The vector includes T4 terminators to ensure proper transcription regulation and can be adapted for use in other systems by swapping out the promoter.
Article Abstract
The pSETT4 vector integrates into the sp. SE50/110 chromosome via the bacteriophage φC31 integrase and allows cloning of a gene of interest by Golden Gate assembly (BsaI). T4 terminators surround the expression cassette to isolate the transcriptional unit and to prevent antisense transcription. The system can be used in other by exchanging the promoter.
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Source |
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7516140 | PMC |
| http://dx.doi.org/10.1128/MRA.00596-20 | DOI Listing |
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pSETT4, an Improved φC31-Based Integrative Vector System for sp. SE50/110.
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Article Synopsis
- The pSETT4 vector uses the φC31 integrase to integrate into the sp. SE50/110 chromosome for efficient gene cloning.
- It employs Golden Gate assembly with the BsaI restriction enzyme to enable easy insertion of the gene of interest.
- The vector includes T4 terminators to ensure proper transcription regulation and can be adapted for use in other systems by swapping out the promoter.
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