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In the past two decades, metabolomics has proved to be a valuable tool with many potential applications in different areas of science. However, there are still some challenges that need to be addressed, particularly for multicenter studies. These challenges are mainly attributed to various sources of fluctuation and unwanted variations that can be introduced at pre-analytical, analytical, and/or post-analytical steps of any metabolomics experiment. Thus, this study aimed at using TK24 as the model organism in a cross-laboratory experiment in Manchester and Leuven to evaluate the reproducibility of a standard sample preparation method, and determine the optimal sample format (cell extract or quenched biomass) required to preserve the metabolic profile of the cells during cross-lab sample transportation and storage. Principal component analysis (PCA) scores plot of the gas chromatography-mass spectrometry (GC-MS) data from both laboratories displayed clear growth-dependent clustering patterns which was in agreement with the Procrustes analysis findings. In addition, the data generated in Manchester displayed tight clustering of cell pellets (quenched biomass) and metabolite extracts, confirming the stability of both sample formats during the transportation and storage period.
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http://dx.doi.org/10.3390/metabo10090379 | DOI Listing |
J Am Chem Soc
December 2024
Department of Chemical and Biological Engineering, Institute of Chemical Processes, Seoul National University, Seoul 08826, Republic of Korea.
Electrochemical liquid-cell transmission electron microscopy (e-LCTEM) offers great potential for investigating the structural dynamics of nanomaterials during electrochemical reactions. However, challenges arise from the difficulty in achieving the optimal electrolyte thickness, leading to inconsistent electrochemical responses and limited spatial resolution. In this study, we present advanced e-LCTEM techniques tailored for tracking Pt/C degradation under electrochemical polarization at short intervals with high spatial resolution.
View Article and Find Full Text PDFEnviron Geochem Health
December 2024
Department of Chemistry, North Carolina State University, Raleigh, NC, USA.
This study investigated the occurrence, concentration and human health risks of five pharmaceutical residues-metronidazole, sulfamethoxazole, ciprofloxacin, carbamazepine, and caffeine-in groundwater and surface water samples from Kampala and Mbarara districts of Uganda. The present study also employed techniques of remote sensing and geographic information system (GIS); thereby, emphasizing the importance of thematic mapping, land use classification, and spatial buffering to evaluate pharmaceutical contaminants in an environmental setting. The risk quotient (RQ) approach was also employed to assess the risk of exposure to the pharmaceutical contaminants.
View Article and Find Full Text PDFTalanta
December 2024
Thin Films and Materials Science Research Laboratory, Department of Physics, Dayanand Science College, Latur, Maharashtra, 413512, India. Electronic address:
In this study, we report the synthesis, optical characterization and ultra-sensitive ammonia gas sensing properties of Mg-doped ZnO cauliflower like nanostructures obtained via chemical spray pyrolysis technique. The morphological and structural properties of the prepared films were investigated by Field Emission Scanning electron microscope (FESEM) and X-ray diffraction (XRD). Gas sensing and optical characterizations were carried out using Keithley electrometer and Uv-Vis.
View Article and Find Full Text PDFNurse Educ Pract
December 2024
Ankara Yıldırım Beyazıt University, Faculty of Health Sciences, Department of Nursing, Ankara, Turkey. Electronic address:
Objectives: This cross-sectional correlational study investigated the relationship between a hidden curriculum and fourth-year nursing students' career plans, professional values, and professional readiness.
Background: The hidden nursing education curriculum can influence professional outcomes. While nursing curriculum is designed to prepare students to become proficient nurses, the hidden curriculum is linked to unintended learning outcomes.
Spectrochim Acta A Mol Biomol Spectrosc
December 2024
Department of Polymeric Materials and Engineering, School of Materials and Energy, Guangdong University of Technology, Guangzhou 510006, China. Electronic address:
High copper levels pose a risk to environmental and human health due to their toxicity and widespread industrial application, in which abnormal copper levels are associated with various diseases both in neurodegenerative diseases and plant growth. Thus, a turn-on fluorescent probe BBYD-Cu, based on donor-acceptor type structure, was designed and synthesized with easy preparations. BBYD-Cu can specifically recognized Cu by 2-picolinic ester group, then released the fluorophore to enhance the fluorescent signals.
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