sp. nov., isolated from the gut of the fungus growing-termite .

The taxonomic position of a novel aerobic, Gram-positive actinobacteria, designated strain RB5, was determined using a polyphasic approach. The strain, isolated from the gut of the fungus-farming termite , showed morphological, physiological and chemotaxonomic properties typical of the genus . Based on 16S rRNA gene sequence analysis, the closest phylogenetic neighbour of RB5 was DSM 42102 (98.87 %). DNA-DNA hybridization experiments between strain RB5 and DSM 42102 resulted in a value of 27.4 % (26.8 %). The cell wall of strain RB5 contained ll-diaminopimelic acid as the diagnostic amino acid. Mycolic acids and diagnostic sugars in whole-cell hydrolysates were not detected. The strain produced the following major phospholipids: diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol-mannoside and phosphatidylserine. The menaquinone profile showed hexa- and octahydrogenated menaquinones containing nine isoprene units [MK-9(H) and MK-9(H)]. The strain exhibited a fatty acid profile containing the following major fatty acids: 12-methyltridecanoic acid (iso-C) 12-methyltetradecanoic acid (anteiso-C), 13-methyltetradecanoic acid (iso-C) and 14-methylpentadecanoic acid (iso-C). Here, we propose a novel species of the genus with the type strain RB5 (=VKM Ac-2839=NRRL B65539).