AI Article Synopsis
- Lipids are essential for various cellular functions, but understanding their movement and roles in cells has been challenging due to limited imaging methods.
- The authors developed a new technique for selectively labeling and visualizing phosphatidylcholine in specific organelles using a metabolic marker and bioorthogonal reactions.
- This approach enabled live-cell imaging that provided direct evidence of how the autophagosomal membrane forms from the endoplasmic reticulum, offering a powerful tool for studying lipid transport in real-time.
Article Abstract
Lipids play crucial roles as structural elements, signaling molecules and material transporters in cells. However, the functions and dynamics of lipids within cells remain unclear because of a lack of methods to selectively label lipids in specific organelles and trace their movement by live-cell imaging. We describe here a technology for the selective labeling and fluorescence imaging (microscopic or nanoscopic) of phosphatidylcholine in target organelles. This approach involves the metabolic incorporation of azido-choline, followed by a spatially limited bioorthogonal reaction that enables the visualization and quantitative analysis of interorganelle lipid transport in live cells. More importantly, with live-cell imaging, we obtained direct evidence that the autophagosomal membrane originates from the endoplasmic reticulum. This method is simple and robust and is thus powerful for real-time tracing of interorganelle lipid trafficking.
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| http://dx.doi.org/10.1038/s41589-020-00651-z | DOI Listing |
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