The study aimed to identify the preference of pathways of humus formation. Five lab-scale composting experiments were established: the control (CK), montmorillonite addition (M), illite addition (I), thermal treatment montmorillonite addition (M-) and thermal treatment illite addition (I-). Results showed humus content was increased by 11.5%, 39.3%, 37.2%, 30.9% and 27.6% during CK, M-, M, I- and I composting. Meanwhile, Redundancy analysis indicated the bands of bacteria community related to humic acid (HA) were more abundant in the M- and I- treatments. Furthermore, structural equation model and variance partitioning analysis demonstrated that M- and I- treatments promoted precursors to synthesize HA by coordinated regulation of biotic pathway and abiotic pathway, the increase of HA in the M and I treatments mainly through the abiotic pathway. In summary, an effective method was proposed to improve humus production by adjusting the preference of biotic and abiotic pathways of humus formation.
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