AI Article Synopsis
- Species from the Celastraceae family contain unique genes that can alter oil content and fatty acid composition in vegetable oils.
- Researchers identified and isolated a gene coding for diacylglycerol acetyltransferase (DAcT) that allows plants to create acetylated triacylglycerols.
- This gene was successfully modified, cloned, and inserted into tobacco plants, leading to confirmed overexpression and variable production of acetylated triacylglycerols in the transgenic plants' immature seeds.
Article Abstract
species from the Celastraceae family are considered as a source of unusual genes modifying the oil content and fatty acid composition of vegetable oils. Due to the possession of genes encoding enzyme diacylglycerol acetyltransferase (DAcT), plants can synthesize and accumulate acetylated triacyglycerols. The gene from () encoding the DAcT was identified, isolated, characterized, and modified for cloning and genetic transformation of plants. This gene has a unique nucleotide sequence and amino acid composition, different from orthologous genes from other species. Nucleotide sequence of original gene was modified, cloned into transformation vector, and introduced into tobacco plants. Overexpression of gene was confirmed, and transgenic host plants produced and accumulated acetylated triacylglycerols (TAGs) in immature seeds. Individual transgenic plants showed difference in amounts of synthesized acetylTAGs and also in fatty acid composition of acetylTAGs.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7554731 | PMC |
| http://dx.doi.org/10.3390/life10090205 | DOI Listing |
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