Two halophilic archaeal strains, C90 and YPL13, were isolated from a salt lake and a salt mine in PR China. The two strains were found to form two clusters (97.5 and 89.5 % similarity between them, respectively) separating them from the three current members of the genus (95.4-97.0 % and 86.6-89.3 % similarity, respectively) on the basis of the 16S rRNA and gene sequence similarities and phylogenetic analysis. Diverse phenotypic characteristics differentiate strains C90 and YPL13 from current members. The polar lipids of strain C90 were phosphatidic acid, phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me), phosphatidylglycerol sulphate, two unidentified glycolipids, a major glycolipid and a minor glycolipid, while those of strain YPL13 were PG, PGP-Me, two unidentified phospholipids and a glycolipid. The average nucleotide identity (ANI) and DNA-DNA hybridization (DDH) values between the two strains were 79.8 and 27.1 %, respectively, which were much lower than the threshold values proposed as a species boundaries (ANI 95-96 % and DDH 70 %), which revealed that the two strains represent two novel species; these values (ANI 76.6-80.0 % and DDH 21.6-27.0 %) of the strains examined in this study and the current members of are much lower than the recommended threshold values, suggesting that strains C90 and YPL13 represent two genomically different species of . These results showed that strains C90 (=CGMCC 1.13738=JCM 32961) and YPL13 (=CGMCC 1.13884=JCM 31111) represent two novel species of , for which the names sp. nov. and sp. nov. are proposed.
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