AI Article Synopsis
- This study examined the importance of a healthy oral microbiome and how to monitor it using advanced diagnostics.
- The research involved 214 participants, grouping them into healthy, caries, and periodontitis categories, based on clinical evaluations and saliva samples.
- A new qPCR assay was developed, revealing that eight out of ten targeted bacteria could effectively distinguish between healthy and periodontitis groups, highlighting the potential of saliva analysis for assessing oral health.
Article Abstract
Oral health is maintained by a healthy microbiome, which can be monitored by state-of-the art diagnostics. Therefore, this study evaluated the presence and quantity of ten oral disease-associated taxa (, , , , , , , , oral associated ) in saliva and their clinical status association in 214 individuals. Upon clinical examination, study subjects were grouped into healthy, caries and periodontitis and their saliva was collected. A highly specific point-of-care compatible dual color qPCR assay was developed and used to study the above-mentioned bacteria of interest in the collected saliva. Assay performance was compared to a commercially available microbial reference test. Eight out of ten taxa that were investigated during this study were strong discriminators between the periodontitis and healthy groups: , , , , , and oral ( < 0.05). Significant differentiation between the periodontitis and caries group microbiome was only shown for ( < 0.05). A clear distinction between oral health and disease was enabled by the analysis of quantitative qPCR data of target taxa levels in saliva.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7565348 | PMC |
| http://dx.doi.org/10.3390/jcm9092945 | DOI Listing |
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