Triterpenoids have been described in Andrographis paniculata. Oleanolic acid exhibits high biological activity and is widely used in the clinic, and β-sitosterol not only has good biological activity but also plays an important physiological role in plants. However, analysis of the biosynthetic pathway of triterpenoids in Andrographis paniculata has not been reported. Here, we provide the first report of the isolation and identification of nine 2, 3-oxidosqualene cyclases (ApOSC3 to ApOSC11) from A. paniculata. The results showed that ApOSC4 represented a monofunctional synthase that could convert 2, 3-oxidosqualene to β-amyrin. ApOSC5 as a bifunctional 2, 3-oxidosqualene cyclases, could transfer 2, 3-oxidosqualene to β-amyrin and α-amyrin. ApOSC6 to ApOSC8 composed the multifunctional 2, 3-oxidosqualene cyclases that could convert 2, 3-oxidosqualene to β-amyrin, α-amyrin and one or two undetermined triterpenoids. This study provides a better understanding of the biosynthetic pathway of triterpenoids in A. paniculata, and the discovery of multifunctional 2, 3-oxidosqualene cyclases ApOSC5 to ApOSC8 of the facilitates knowledge of the compounds diversity in A. paniculata.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/S1875-5364(20)60004-8 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Discovery and Functional Identification of 2,3-Oxidosqualene Cyclases and Cytochrome P450s in Triterpenoid Metabolic Pathways of .
J Agric Food Chem
December 2024
Guangdong Engineering Research Center of Biosynthesis and Metabolism of Effective Components of Chinese Medicine, International Institute for Translational Chinese Medicine, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, P. R. China.
Article Synopsis
- * The study identified key enzyme genes involved in the triterpenoid metabolic pathways using transcriptome sequencing and synthetic biology, particularly focusing on two 2,3-oxidosqualene cyclases and two cytochrome P450s.
- * Researchers successfully reconstructed the biosynthetic pathway for ursane and oleanane-type triterpenoids in a yeast host, detailing the enzymatic reactions necessary for producing important compounds like ursolic acid and oleanolic acid.
Identification, functional characterization and expression profiling of three triterpene synthases from the legume plant Vigna unguiculata.
Biochem Biophys Res Commun
December 2024
Department of Biochemistry and Biotechnology, Plant and Environmental Biotechnology Laboratory, University of Thessaly, Viopolis, Larissa, 41500, Greece. Electronic address:
Oxidosqualene cyclases (OSCs) are important regulatory enzymes involved in cyclization reactions of 2, 3-oxidosqualene to form triterpenes and sterols. This study presents the identification and characterization of three OSC genes, a β - amyrin synthase (VuβAS), a lupeol synthase (VuLUS) and a cycloartenol synthase (VuCAS) in Vigna unguiculata, an edible leguminous plant with high nutritional and nutraceutical value. Phylogenetic analysis showed that the VuβAS, VuLUS and VuCAS were clustered within the clades of previously characterized β - amyrin synthases, lupeol synthases and cycloartenol synthases.
View Article and Find Full Text PDFFunctional diversity of oxidosqualene cyclases in genus Oryza.
New Phytol
December 2024
Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.
Triterpene skeletons, catalyzing by 2,3-oxidosqualene cyclases (OSCs), are essential for synthesis of steroids and triterpenoids. In japonica rice cultivars Zhonghua11, a total of 12 OsOSCs have been found. While the catalytic functions of OsOSC1, 3, 4, 9, and 10 remain unclear, the functions of the other OsOSCs have been well studied.
View Article and Find Full Text PDF[Cloning and functional characterization of oxidosqualene cyclase gene for α-amyrin synthesis].
Zhongguo Zhong Yao Za Zhi
June 2024
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
Article Synopsis
- Ursolic acid is gaining attention for its pharmacological properties and market potential, but current extraction methods from loquat leaves are inefficient and costly.
- A biosynthesis approach using α-amyrin as a precursor offers a promising alternative, as its yield is linked to ursolic acid production.
- Researchers identified and cloned four enzymes from different plants capable of producing α-amyrin, with AaOSC2 showing the highest efficiency, which can help in developing engineered yeast strains for enhanced ursolic acid production.
Integrated Metabolomics and Transcriptomics Analysis Reveals New Insights into Triterpene Biosynthesis in .
Plants (Basel)
June 2024
College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, China.
is highly regarded for its aesthetic and therapeutic qualities. In particular, 's flowers are known to produce essential oils containing a mixture of volatile terpenes, phenylpropanoids, and other compounds. Despite this, extensive research exists on volatile terpenes in flowers, while the knowledge of non-volatile terpenes in distinct tissues is still limited.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!