Objective: To establish an analytical method for determination of 6 kinds of α_2-agonists in animal foods by ultra high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).
Methods: The samples of animal food were enzymatic hydrolysis by β-glucosidase/arylsulfatase, purified by MCX column. The separation was performed on a Dikma leapsil C_(18) column(2. 1 mm×100 mm, 2. 7 μm), then the target compound were detected by ultra high performance liquid chromatography-tandem mass spectrometry with electron spray ionization(ESI) positive ion scan in mode of multiple reaction monitoring(MRM) and quantified by matrix matched external standard method.
Results: At the spiked level of 1, 2 and 4 μg/kg, the recoveries of each compound were in the range of 70. 4%-111. 2% with the relative standard deviations of 2. 3%-18. 8%. The qualitative limits of detections were 0. 06-0. 3 μg/kg and the quantitative limits were 0. 2-1. 0 μg/kg for the 6 targets compounds. By using the established method, the target compound in 30 samples including pork, pig liver, pig kidney, beef and mutton were detected, and no excessive veterinary drug residue were detected.
Conclusion: The established method is simple, rapid, high sensitivity and good stability, with a wide variety and a certain development. It can provide more convenient and fast detection method support for the daily monitoring of veterinary drug residues in animal food.
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| http://dx.doi.org/10.19813/j.cnki.weishengyanjiu.2020.04.012 | DOI Listing |
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