AI Article Synopsis
- The CRISPR/Cas systems are revolutionizing gene editing in lactic acid bacteria (LAB) due to their high efficiency, convenience, and versatility.
- Cas-RNA cassettes are being used for various genetic modifications, including gene deletion, insertion, and point mutations in LAB species.
- The article reviews the basic functions of CRISPR-Cas systems, current editing methods for LAB, and compares different genomic manipulation techniques based on Cas proteins and recombineering types, highlighting the fast-paced advancements in this field.
Article Abstract
The high efficiency, convenience and diversity of clustered regular interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems are driving a technological revolution in the gene editing of lactic acid bacteria (LAB). Cas-RNA cassettes have been adopted as tools to perform gene deletion, insertion and point mutation in several species of LAB. In this article, we describe the basic mechanisms of the CRISPR-Cas system, and the current gene editing methods available, focusing on the CRISPR-Cas models developed for LAB. We also compare the different types of CRISPR-Cas-based genomic manipulations classified according to the different Cas proteins and the type of recombineering, and discuss the rapidly evolving landscape of CRISPR-Cas application in LAB.
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| http://dx.doi.org/10.1007/s11033-020-05820-w | DOI Listing |
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