The control of cell-microenvironment interactions plays a pivotal role in constructing specific scaffolds for tissue engineering. Here, we fabricated a 3D free-standing ordered graphene (3D-OG) network with a precisely defined pattern. When primary cortical cells are cultured on 3D-OG scaffolds, they form well-defined 3D connections. Astrocytes have a more ramified shape similar to that seen because of the nanosized ripples and wrinkles on the surface of graphene skeleton. Neurons have axons and dendrites aligned along the graphene skeleton, allowing the formation of neuronal networks with highly controlled connections. Neuronal networks have higher electrical activity with functional signaling over a long distance along the graphene skeleton. Our study, for the first time, investigated the geometrical cues on ordered neuronal growth and network formation with the support of graphene in 3D, which therefore advanced the development of customized scaffolds for brain-machine interfaces or neuroprosthetic devices.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/acs.nanolett.0c02107 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Anti-Fatigue Cellular Graphene Aerogel Through Multiscale Joint Strengthening.
Adv Mater
December 2024
Laboratory for Multiscale Mechanics and Medical Science, SV LAB, School of Aerospace, Xi'an Jiaotong University, Xi'an, 710049, China.
Despite fatigue free of monolayer graphene, its assemblies, like cellular graphene aerogels (CGA), are usually suffering of frequent fatigue and inherent strength degradation in repeated loading. In this work, by employing multiscale modeling, the highly intrinsic anisotropic mechanical properties of the cell wall due to the layer-by-layer stacked graphene sheets are uncovered, which easily trigger the unique skeleton joints damage during repeated loading and contribute the primary fatigue mechanism of CGA. Conversely, multiscale joint strengthening strategies are proposed by interlayer crosslinking and joint curvation, improving the interlayer interaction, and decreasing interlayer stress during compression, respectively, so as to effectively suppress joint damage to improve fatigue performance of CGA.
View Article and Find Full Text PDFIn Situ Laser Direct Writing of Graphene-Based Layered Hybrid Materials with Superhydrophilicity.
ACS Appl Mater Interfaces
December 2024
State Key Laboratory of Polymer Materials Engineering of China, Polymer Research Institute, Sichuan University, Chengdu 610065, China.
Laser-induced graphene (LIG) has attracted extensive attention as an electrode material. However, it usually exhibits limited electrochemical performance in many applications due to the limited electrical conductivity and charge storage properties. Herein, we proposed a simple and environmentally friendly strategy for in situ preparation of flexible Au/LIG/PI layered hybrid materials using laser direct writing.
View Article and Find Full Text PDFEarly Diagnosis of Tumorigenesis via Ratiometric Carbon Dots with Deep-Red Emissive Fluorescence Based on NAD Dependence.
Molecules
November 2024
College of Chemistry, Zhengzhou University, Zhengzhou 450001, China.
The early diagnosis of tumorigenesis is crucial for clinical treatment, but the resolution and sensitivity of conventional short-wavelength biomarkers are not ideal because of the complicated interference in living tissue. Herein, a nicotinamide adenine dinucleotide (NAD)-responsive probe with deep-red emissive ratiometric fluorescence was synthetized as a promising target for energy metabolism patterns during tumorigenesis. Interestingly, the solvents HPO and 2,2'-dithiodibenzoic acid enhanced the red emission (640 and 680 nm) of o-phenylenediamine-based carbon dots (CDs), leading to the formation of a nanoscale graphite-like skeleton covered with -P=O, -CONH-, -COOH and -NH on their surfaces.
View Article and Find Full Text PDFCytoskeletal regulation on polycaprolactone/graphene porous scaffolds for bone tissue engineering.
Sci Rep
November 2024
Center of Translational Oral Research, University of Bergen, Bergen, 5262, Norway.
Understanding cellular mechanics requires evaluating the mechanical and chemical cues that regulate the actin cytoskeleton, particularly filopodia and lamellipodia. Therefore, this study aims to investigate the effect of scaffolds properties on cell migration. The results showed that scaffolds toughness, strain, and strength played a key role in promoting cell movement by stimulating the dynamic formation of filopodia and lamellipodia.
View Article and Find Full Text PDFA novel multifunctional nanocomposite hydrogel orchestrates the macrophage reprogramming-osteogenesis crosstalk to boost bone defect repair.
J Nanobiotechnology
November 2024
Department of Orthopedics and Department of Plastic Surgery, The First Affiliated Hospital of Bengbu Medical University, Bengbu, 233004, China.
Repairing bone defects is a complex cascade reaction process, as immune system regulation, vascular growth, and osteogenic differentiation are essential. Thus, developing a tissue-engineered biomaterial that caters to the complex healing process of bone regeneration remains a major clinical challenge. In the study, Ca-TA-rGO (CTAG)/GelMA hydrogels were synthesized by binding Ca using metal chelation to graphene oxide (GO) nanosheets reduced by tannic acid (TA-rGO) and doping them into gelatin methacrylate (GelMA) hydrogels.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!