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Gene expression profile of epithelial-mesenchymal transition mediators in papillary thyroid cancer. | LitMetric

AI Article Synopsis

  • The study investigates how platelet derived growth factor receptor alpha (PDGFRA) affects the epithelial-mesenchymal transition (EMT) in thyroid cells and its connection to metastatic progression in papillary thyroid cancer (PTC).
  • Researchers conducted transcriptional analysis of thyroid tumors and surrounding tissues using a TaqMan Low Density array, revealing key gene expression changes associated with PDGFRA and EMT. The findings indicated that genes like DPP4 and SPP1 were upregulated in metastatic disease, while TFF3 and SOX10 were downregulated.
  • The results suggest that PDGFRA not only promotes EMT but also impairs epithelial integrity by altering gene expression, increasing cell motility,

Article Abstract

Purpose: Platelet derived growth receptor alpha (PDGFRA) promotes the epithelial-mesenchymal transition (EMT) in thyroid follicular cells and is linked to lymphatic metastases in papillary thyroid cancer (PTC). We probed the regulatory network of genes linked to PDGFRA and EMT, comparing matched patient primary tumor and metastatic specimens, as well as engineered cell lines and ex vivo primary cultures with and without PDGFRA.

Methods: Freshly isolated thyroid tumors with or without metastases, with matching neighboring benign or normal tissue, was isolated for comparative transcriptional analysis using a TaqMan Low Density array (TLDA) assay with genes representing important markers of EMT, cellular adhesion, apoptosis, differentiation, senescence, and signal transduction pathways in thyroid cancer. Transfected primary cultures and immortalized cell lines were also analyzed with respect to PDGFRA expression and cell phenotype.

Results: We reveal the consistent upregulation of serine protease DPP4 and structural protein SPP1 with the progression of PTC to metastatic disease, as well as with PDGFRA expression. Conversely, epithelial integrity gene TFF3 and transcription factor SOX10 were strongly down-regulated. This gene network also includes important mediators of EMT including DSG1, MMP3, MMP9, and BECN. We observed similar genomic changes in ex vivo normal thyroid cells transfected with PDGFRA that also exhibited a partially dedifferentiated phenotype. In particular, we observed lamellopodia with induction of PDGFRA and illustrate that DPP4 and SPP1 were upregulated in this process, with decreased TFF3 and SOX10 as seen in tissue specimens. PDGFRA did decrease nuclear protein levels of differentiation factor TTF1, but not the transcription of TTF1 and PAX8.

Conclusions: We demonstrate that PDGFRA activates EMT pathways and decreases expression of genes favoring epithelial integrity, pushing follicular cells toward a dedifferentiated phenotype. SPP1 and DPP4, previously linked with adverse outcomes in thyroid cancer, appear to be regulated by PDGFRA. PDGFRA expression promotes metastatic disease through multiple EMT levers that favor formation of an invasive phenotype and increased metalloproteinase expression.

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Source
http://dx.doi.org/10.1007/s12020-020-02466-3DOI Listing

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