Sapovirus are important agents of acute gastroenteritis (AGE) and they are associated with outbreaks and sporadic cases worldwide. They infect people of all ages, but mainly children, the elderly and immunocompromised individuals are affected. The aim of this study was investigate sapovirus and to determine viral loads in fecal samples from patient undergoing allogeneic hematopoietic stem cell transplantation (allo-HSCT). Fecal samples were submitted to extraction of the genetic material using a commercial kit, and RT-qPCR TaqMan was used for sapovirus screening and determination of viral loads, using a standard curve with serial dilutions of a recombinant plasmid. Positive samples were sequence by Sanger method. Sapovirus was detected in one patient, 5.3% (1/19). Viral excretion lasted for 16 days. Viral load varied from 1.73 × 10 to 8.97 × 10 GC/g. One of the positive samples was characterized as GI.1 genotype. This is the first study to determine sapovirus loads in samples from allo-HSCT and to identify GI.1 genotype in immunocompromised patients.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7459042 | PMC |
| http://dx.doi.org/10.1007/s13337-020-00583-1 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Water quality assessment of Johor River Basin, Malaysia, using multivariate analysis and spatial interpolation method.
Environ Sci Pollut Res Int
January 2025
Center for Environmental Sustainability and Water Security (IPASA), Research Institute for Sustainable Environment (RISE), Universiti Teknologi Malaysia, 81310, Johor Bahru, Johor, Malaysia.
In the Johor River Basin, a comprehensive analysis was conducted on 24 water environmental parameters across 33 sampling sites over 3 years, encompassing both dry and wet seasons. A total of 396 water samples were collected and analyzed to calculate the Water Quality Index (WQI). To further assess water quality and pinpoint potential pollution sources, multivariate techniques such as principal component analysis (PCA) and cluster analysis (CA), alongside spatial analysis using inverse distance weighted (IDW) interpolation, were employed.
View Article and Find Full Text PDFPersistence of human Aichi virus infectivity from raw surface water to drinking water.
Appl Environ Microbiol
December 2024
Centre National de Référence des virus des gastro-entérites, Centre Hospitalier Universitaire Dijon Bourgogne, Dijon, France.
Human Aichi virus 1 (AiV-1) is a water- and food-borne infection-associated picornavirus that causes gastroenteritis in humans. Recent studies on environmental waters showed a high frequency and abundance of AiV-1, suggesting that it might be an appropriate indicator of fecal contamination. We screened 450 surface and drinking water samples from a Tunisian drinking water treatment plant (DWTP) and the Sidi Salem dam for AiV-1 by real time reverse transcriptase PCR (RT-qPCR).
View Article and Find Full Text PDFThe prebiotic potential of lactobin and glucans combined with goat's milk in cats.
Can J Vet Res
January 2025
Key Laboratory of Feed Biotechnology, Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, No. 12 Zhong Guan Cun South Street, Haidian District, Beijing 100081, China (Han, Sun, Gu, J. Wang, X. Wang, Tao, Z. Wang, Liu); Jiangxi Agricultural University, No. 1225, Zhimin Avenue, Xinjian District, Nanchang City 330045, China (Gu).
Prebiotics are important for gut health and immunity in animals and could promote the growth of beneficial bacteria. The objective of this study was to investigate the prebiotic potential of lactobin and glucans when combined with goat's milk in the diet of cats. Twenty-four healthy cats (all about 2 y old) were randomly assigned to 4 treatment groups.
View Article and Find Full Text PDFPreliminary report: Protective effects of probiotics on cefovecin-induced gut dysbiosis in dogs.
Can J Vet Res
January 2025
Laboratory of Veterinary Dermatology (Han, Hwang) and Research Institute for Veterinary Science (Han, Mun, Hwang), College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea; ORIGIN Veterinary Dermatology Clinic, Busan, Republic of Korea (Kang); Department of Companion Animal Health Care, College of Medical Health, Kyungbok University, Namyangju, Republic of Korea (Kim S-J); Department of Large Animal Internal Medicine, College of Veterinary Medicine, Kangwon National University, Chuncheon, Republic of Korea (Kim Y-H).
The objective of this study was to evaluate whether supplementation with probiotics over a 2-week period stabilizes the gut microbiota in dogs following prolonged cefovecin treatment. A significant number of clinical veterinarians prescribe oral probiotics to dogs in conjunction with systemic antibiotics with the intention of protecting against gut dysbiosis. The effects of antibiotics and probiotics in dogs have not been extensively studied, however, and the optimal treatment for gut dysbiosis remains uncertain.
View Article and Find Full Text PDFTumor immunomodulation by nanoparticle and focused ultrasound alters gut microbiome in a sexually dimorphic manner.
Theranostics
January 2025
Department of Radiation Oncology, UT Southwestern Medical Center, Dallas, TX 75390, USA.
Local immunomodulation with nanoparticles (NPs) and focused ultrasound (FUS) is recognized for triggering anti-tumor immunity. However, the impact of these tumor immunomodulations on sex-specific microbiome diversity at distant sites and their correlation with therapeutic effectiveness remains unknown. Here, we conducted local intratumoral therapy using immunogenic cell death-enhancing Calreticulin-Nanoparticles (CRT-NPs) and FUS in male and female mice.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!