Emergence of Harboring ST29 and ST11 in Veterinary Settings and Waste of Pakistan.

Introduction: Intense livestock farming practices enforcing the farmers to use antibiotics as food supplements on a routine basis. Aberrant use of antibiotics is associated with the emergence of antibiotics resistance and resistant superbugs. Keeping in view the current scenario, the present study was designed for the first time from Pakistan with a specific aim to estimate the prevalence of the carbapenem-resistant in veterinary settings and the waste in Pakistan.

Methods: A total of 138 samples from various veterinary sources were collected by employing a nonprobability sampling technique. Isolation and phenotypic identification of carbapenem-resistant were performed according to the CLSI standard. Molecular detection of various antibiotic resistance genes (ARGs) was done through PCR by using specific primers against each ARG. According to the pasture scheme, the multilocus sequence typing (MLST) was performed to characterize the sequence types (STs).

Results: According to the results of the study, overall 9.4% (13/138) isolates were confirmed carbapenem-resistant . Among various carbapenem ARGs particularly, the was found in 92.3% (12/13) isolates followed by 84.6% (11/13). MLST results revealed that overall 3 STs were found in the study which includes ST29, ST11, and ST258. Taking together, this is the first study to our best knowledge which demonstrated the prevalence of carbapenem-resistant and its various STs prevalent in veterinary settings and the waste of Pakistan.

Conclusion: Based on the above-mentioned facts, we suggested that veterinary settings and waste are the potential source and reservoir of carbapenem-resistant , which may be disseminated to the environment and ultimately can affect the public and companion livestock health.