AI Article Synopsis
- The immune synapse (IS) is a critical site for communication between immune cells, helping to activate T-cells and secrete vesicles through close contact and molecular interactions.
- The formation and signaling of the IS rely on the polarization of organelles, guided by the cytoskeleton, leading to important vesicular traffic.
- The text describes a method to analyze the rapid localization changes of vesicles using microscopy, utilizing the centrosome and microtubules or actin structures as reference points during IS organization.
Article Abstract
The immune synapse (IS) enables cell-cell communication between immune cells through close contacts, as well as T-cell activation and vesicle secretion. It is sustained by fine-tuned molecular interactions of receptors at both cell sides of the IS and intracellular cytoskeletal components. The resulting intracellular polarization of different organelles, through cytoskeleton-guided vesicular traffic, is a key player in IS formation and signaling. We describe herein a method to analyze rapid changes of vesicle localization through microscopy analysis upon polarization toward the IS. These vesicles are monitored using the centrosome and its associated microtubular network or the actin-based structures as spatial references during the organization of the IS.
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| http://dx.doi.org/10.1007/7651_2020_321 | DOI Listing |
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