The pollution of antibiotics in aquaculture environment is increasingly serious, and excessive antibiotics will kill the probiotics in aquaculture feed. How to improve the viability of probiotics in the antibiotics-contaminated environment is of significance. In this study, a new strategy for protecting Saccharomyces cerevisiae cells in situ against antibiotics is constructed based on cell surface engineering technology by putting on wearable protective layers for cells. The protective layer is constructed around cellular surface via the self-assembly of coacervate microdroplets that consist of carboxymethyl chitosan and carboxyl dextran. Without affecting the cell viability, the protective layer can grasp ciprofloxacin and decrease the contact of ciprofloxacin to cells and consequently improve the survival rate of cells when exposing to ciprofloxacin. This work highlights a facile strategy to establish removable artificial cell wall by biodegradable polysaccharides for improving the productivity of probiotics in antibiotic environments.
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| http://dx.doi.org/10.1002/mabi.202000185 | DOI Listing |
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