AI Article Synopsis

  • Researchers developed a new red-shifted dopamine sensor called RdLight1, which allows for high-resolution imaging of dopamine dynamics in animals while avoiding interference with existing green fluorescent protein (GFP)-based sensors. !* -
  • RdLight1 demonstrates excellent photostability and can be utilized for receptor-specific pharmacological studies, tracking dopamine release, and monitoring neuronal activity in live animals. !* -
  • The study found that dopamine release in the nucleus accumbens triggered by reward-predictive cues results in a quick suppression of glutamate release, highlighting RdLight1's potential for exploring complex interactions within neural circuits. !*

Article Abstract

Genetically encoded dopamine sensors based on green fluorescent protein (GFP) enable high-resolution imaging of dopamine dynamics in behaving animals. However, these GFP-based variants cannot be readily combined with commonly used optical sensors and actuators, due to spectral overlap. We therefore engineered red-shifted variants of dopamine sensors called RdLight1, based on mApple. RdLight1 can be combined with GFP-based sensors with minimal interference and shows high photostability, permitting prolonged continuous imaging. We demonstrate the utility of RdLight1 for receptor-specific pharmacological analysis in cell culture, simultaneous assessment of dopamine release and cell-type-specific neuronal activity and simultaneous subsecond monitoring of multiple neurotransmitters in freely behaving rats. Dual-color photometry revealed that dopamine release in the nucleus accumbens evoked by reward-predictive cues is accompanied by a rapid suppression of glutamate release. By enabling multiplexed imaging of dopamine with other circuit components in vivo, RdLight1 opens avenues for understanding many aspects of dopamine biology.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8169200PMC
http://dx.doi.org/10.1038/s41592-020-0936-3DOI Listing

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