AI Article Synopsis
- The Lon gene from E. coli was cloned into the plasmid pBR327, and its complete nucleotide sequence has been determined.
- The cloned gene lacks a terminal codon and is shorter than the original.
- Despite these modifications, the gene still effectively expresses a modified La proteinase that maintains ATP-dependent proteolytic activity.
Article Abstract
Lon gene of E. coli has been cloned into the plasmid pBR327. Full nucleotide sequence of the gene has been established. It was shown that the cloned gene does not possess the terminal codon and is somewhat shortened. Nevertheless it retains full phenotypic activity and expresses the C-end modified La proteinase which retains ATP-dependent proteolytic activity.
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