AI Article Synopsis
- The enteric nervous system (ENS) plays a crucial role in intestinal functions but has been challenging to study due to the rare and diverse nature of its cells.
- Researchers developed two innovative methods, RAISIN RNA-seq and MIRACL-seq, to analyze individual cells in the ENS of adult mice and humans, revealing a high diversity of neurons and significant gene expression changes related to age and disease.
- The study highlighted differences in neuronal profiles between the intestine regions and found that the human ENS expresses genes linked to various diseases, indicating a potential role of these neurons in health issues.
Article Abstract
The enteric nervous system (ENS) coordinates diverse functions in the intestine but has eluded comprehensive molecular characterization because of the rarity and diversity of cells. Here we develop two methods to profile the ENS of adult mice and humans at single-cell resolution: RAISIN RNA-seq for profiling intact nuclei with ribosome-bound mRNA and MIRACL-seq for label-free enrichment of rare cell types by droplet-based profiling. The 1,187,535 nuclei in our mouse atlas include 5,068 neurons from the ileum and colon, revealing extraordinary neuron diversity. We highlight circadian expression changes in enteric neurons, show that disease-related genes are dysregulated with aging, and identify differences between the ileum and proximal/distal colon. In humans, we profile 436,202 nuclei, recovering 1,445 neurons, and identify conserved and species-specific transcriptional programs and putative neuro-epithelial, neuro-stromal, and neuro-immune interactions. The human ENS expresses risk genes for neuropathic, inflammatory, and extra-intestinal diseases, suggesting neuronal contributions to disease.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8358727 | PMC |
| http://dx.doi.org/10.1016/j.cell.2020.08.003 | DOI Listing |
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