Spermatogenesis and steroidogenesis disruption in a model of metabolic syndrome rats.

Context: Metabolic syndrome (MetS) is a clustering of several physiological alterations.

Objective: This study was designed to evaluate the effects of MetS on rats spermatogenesis and steroidogenesis.

Materials And Methods: We developed a MetS rodent model using high-sugar and high-fat diet.

Results: MetS rats showed severe disorders in sperm parameters. Interestingly, a significant increase in malondialdehyde level and a decrease in the antioxidant activities were observed. Moreover, qRT-PCR analysis showed Bax down-regulation and Bcl-2 up-regulation. A decrease in testosterone level was identified, correlated with the , and testicular marker down-regulation. Finally, MetS rats showed an up-regulation of pro-inflammatory cytokines receptors and .

Conclusion: MetS induced severe testis toxicity in male rats. Mets markedly distorted sperm parameters, inhibited the transcription of steroidogenic enzymes and led to oxidative stress, inflammation, and alteration of Bax/Bcl-2 ratioin testicular tissues.