Targeted delivery of β-glucosidase-loaded magnetic nanoparticles: effect of external magnetic field duration and intensity.

The effect of applied magnetic field duration and intensity on the delivery of β-glucosidase-loaded magnetic nanoparticles was evaluated. The prepared β-glucosidase-loaded magnetic nanoparticles were targeted to subcutaneous tumors with an external magnetic field. Iron concentration and enzyme activity in tumor tissue were analyzed via electron spin resonance detection, Prussian blue staining and enzyme activity measurement. The increase in magnetic nanoparticles quantity and enzyme activity in tumor tissue was not synchronous with the magnetic targeting duration. In addition, accumulation of magnetic nanoparticles and the increase in enzyme activity were not synchronous with the magnetic field intensity. The results suggested that appropriate magnetic field conditions should be considered for targeted delivery of bioactivity proteins based on magnetic nanoparticles.